Publication detail
Tensegrity finite element models of mechanical tests of individual cells
BURŠA, J. HOLATA, J. LEBIŠ, R.
Czech title
Tensegritní konečnoprvkové modely mechanických zkoušek izolovaných buněk
English title
Tensegrity finite element models of mechanical tests of individual cells
Type
journal article - other
Language
en
Original abstract
A three-dimensional finite element model of a vascular smooth muscle cell is based on models published recently; it comprehends elements representing cell membrane, cytoplasm and nucleus, and a complex tensegrity structure representing the cytoskeleton. In contrast to previous models of eucaryotic cells, this tensegrity structure consists of several parts. Its external and internal parts number 30 struts, 60 cables each, and their nodes are interconnected by 30 radial members; these parts represent cortical, nuclear and deep cytoskeletons, respectively. This arrangement enables us to simulate load transmission from the extracellular space to the nucleus or centrosome via membrane receptors (focal adhesions); the ability of the model was tested by simulation of some mechanical tests with isolated vascular smooth muscle cells. Although material properties of components defined on the basis of the mechanical tests are ambiguous, modelling of different types of tests has shown the ability of the model to simulate substantial global features of cell behaviour, e.g. action at a distance effect or the global load-deformation response of the cell under various types of loading. Based on computational simulations, the authors offer a hypothesis explaining the scatter of experimental results of indentation tests.
Czech abstract
Je představen 3D konečnoprvkový model hladké svalové buňky, v němž je cytoskelet modelován pomocí tensegritní struktury s 210 prvky. Tento model je využit k simulaci zkoušky tahem a zkoušky průniku, materiálové parametry jsou nastaveny na základě výsledků experimentů.
English abstract
A three-dimensional finite element model of a vascular smooth muscle cell is based on models published recently; it comprehends elements representing cell membrane, cytoplasm and nucleus, and a complex tensegrity structure representing the cytoskeleton. In contrast to previous models of eucaryotic cells, this tensegrity structure consists of several parts. Its external and internal parts number 30 struts, 60 cables each, and their nodes are interconnected by 30 radial members; these parts represent cortical, nuclear and deep cytoskeletons, respectively. This arrangement enables us to simulate load transmission from the extracellular space to the nucleus or centrosome via membrane receptors (focal adhesions); the ability of the model was tested by simulation of some mechanical tests with isolated vascular smooth muscle cells. Although material properties of components defined on the basis of the mechanical tests are ambiguous, modelling of different types of tests has shown the ability of the model to simulate substantial global features of cell behaviour, e.g. action at a distance effect or the global load-deformation response of the cell under various types of loading. Based on computational simulations, the authors offer a hypothesis explaining the scatter of experimental results of indentation tests.
Keywords in Czech
biomechanika buňky; tensegritní struktura; cytoskeleton; výpočtový model
Keywords in English
Cell biomechanics; Tensegrity structure; Cytoskeleton; Computational model
RIV year
2012
Released
16.04.2012
Publisher
Elsevier Science B.V.
Location
Amsterdam
ISSN
0928-7329
Volume
20
Number
2
Pages from–to
135–150
Pages count
16
BIBTEX
@article{BUT88700,
author="Jiří {Burša} and Jakub {Holata} and Radek {Lebiš},
title="Tensegrity finite element models of mechanical tests of individual cells",
year="2012",
volume="20",
number="2",
month="April",
pages="135--150",
publisher="Elsevier Science B.V.",
address="Amsterdam",
issn="0928-7329"
}