Publication detail

Antigen retrieval and fixation of sections on slides for immunohistochemical detection of soya protein in meat products

Bednářová, M. Pospiech, A. Tremlová, B. Řezáčová-Lukášková, Z. Bednář, J.

English title

Antigen retrieval and fixation of sections on slides for immunohistochemical detection of soya protein in meat products

Type

journal article in Web of Science

Language

en

Original abstract

Soybean protein is added to meat products as a cheaper substitute of muscle proteins. If the presence of soybeanprotein in meat products is not stated on the label, this can be taken as adulteration. Detection of soybean proteins in foodstuffs is important in case of a higher rate of their application. This work was focused on the influence of solutions acting as unmasking buffers for soybean antigen retrieval in immunohistochemical examination of meat products. Seven buffers (solutions) with different pH, chemical composition or molarity were tested. The citrate EDTA (ethylene diaminetetraacetic acid) buffer facilitated the highest efficiency in soybean antigen retrieval, while 5% (w/w) urea + H2O the lowest one. Simultaneously, the effect of these buffers on section fixation on slides was tested. The highest efficiency for fixation of sections on slides was recorded for 4% (w/w) AlCl3 + Tris-HCl (tris(hydroxymethyl)aminomethane-HCl) (p < 0.05), which reached the lowest efficiency in terms of antigen retrieval. The efficiency of citrate buffer in antigen retrieval (immunolabelling intensity) was strong and fixation of tissue sections on slides was high (p < 0.05). Therefore, citrate buffer was found to be optimal and can be recommended for immunohistochemistry of meat products.

English abstract

Soybean protein is added to meat products as a cheaper substitute of muscle proteins. If the presence of soybeanprotein in meat products is not stated on the label, this can be taken as adulteration. Detection of soybean proteins in foodstuffs is important in case of a higher rate of their application. This work was focused on the influence of solutions acting as unmasking buffers for soybean antigen retrieval in immunohistochemical examination of meat products. Seven buffers (solutions) with different pH, chemical composition or molarity were tested. The citrate EDTA (ethylene diaminetetraacetic acid) buffer facilitated the highest efficiency in soybean antigen retrieval, while 5% (w/w) urea + H2O the lowest one. Simultaneously, the effect of these buffers on section fixation on slides was tested. The highest efficiency for fixation of sections on slides was recorded for 4% (w/w) AlCl3 + Tris-HCl (tris(hydroxymethyl)aminomethane-HCl) (p < 0.05), which reached the lowest efficiency in terms of antigen retrieval. The efficiency of citrate buffer in antigen retrieval (immunolabelling intensity) was strong and fixation of tissue sections on slides was high (p < 0.05). Therefore, citrate buffer was found to be optimal and can be recommended for immunohistochemistry of meat products.

Keywords in English

unmasking; buffer; epitope; immunolabelling

RIV year

2015

Released

31.03.2015

ISSN

1336-8672

Volume

54

Number

1

Pages from–to

1–8

Pages count

8

BIBTEX


@article{BUT114086,
  author="Josef {Bednář},
  title="Antigen retrieval and fixation of sections on slides for immunohistochemical detection of soya protein in meat products",
  year="2015",
  volume="54",
  number="1",
  month="March",
  pages="1--8",
  issn="1336-8672"
}