Publication detail

Measurement of Fiber Deposition in a Human Lung Model by Phase Contrast Microscopy with Automated Image Analysis

LÍZAL, F. ELCNER, J. BĚLKA, M. JEDELSKÝ, J. HOPKE, P. ŠTARHA, P. DRUCKMÜLLEROVÁ, H. JÍCHA, M.

Czech title

Měření depozice vláken v modelu lidských plic metodou fázově kontrastní mikroskopie s automatickou analýzou obrazu

English title

Measurement of Fiber Deposition in a Human Lung Model by Phase Contrast Microscopy with Automated Image Analysis

Type

journal article - other

Language

en

Original abstract

Deposition of fibers in human lungs is known as a health hazard. In-vitro measurements were performed with monodisperse glass fibers in a realistic model of human lungs up to seventh generation of branching to estimate the effect of fiber size and breathing pattern on fiber deposition. Deposited fibers were rinsed from the model segments and gathered on nitrocellulose filters. Phase-contrast microscopy with high resolution camera was used to capture images of filters with fibers. New software was developed for an automated image analysis and local deposition characteristics were calculated afterwards. The whole method proved to be a useful and valuable tool for the evaluation of fiber and particle deposition.

Czech abstract

Depozice vláknových částic v lidském dýchacím ústrojí představuje známé zdravotní riziko. In-vitro měření byla provedena za pomoci monodispersních skleněných vláken v realistickém modelu lidských plic do sedmé generace větvení pro určení vlivu vláken a jejich velikosti na režim dýchání a depozici v plicích. Usazená vlákna byla dále vymyta ze segmentů modelu a zachycena na filtrech. Fázová kontrastní microskopie s kamerou s vysokým rozlišením byla použita při nasnímání vláken usazených na filtrech. Dále byl vyvinut software pro automatizaci analýzy nasnímaných obrazů pro lepší výpočet depozice skleněných vláken. Tato metoda se jeví jako užitečná a hodnotná pro stanovování depozice vláken a tuhých částic.

English abstract

Deposition of fibers in human lungs is known as a health hazard. In-vitro measurements were performed with monodisperse glass fibers in a realistic model of human lungs up to seventh generation of branching to estimate the effect of fiber size and breathing pattern on fiber deposition. Deposited fibers were rinsed from the model segments and gathered on nitrocellulose filters. Phase-contrast microscopy with high resolution camera was used to capture images of filters with fibers. New software was developed for an automated image analysis and local deposition characteristics were calculated afterwards. The whole method proved to be a useful and valuable tool for the evaluation of fiber and particle deposition.

Keywords in Czech

Lidské plíce, Depozice, Vlákna, Analýza obrazu

Keywords in English

Human Lungs, Deposition, Fibers, Image analysis

RIV year

2013

Released

20.12.2013

Publisher

Association for Engineering Mechanics

Location

Brno

ISSN

1802-1484

Volume

20

Number

3/4

Pages from–to

187–194

Pages count

8

BIBTEX


@article{BUT96300,
  author="František {Lízal} and Jakub {Elcner} and Miloslav {Bělka} and Jan {Jedelský} and Philip K. {Hopke} and Pavel {Štarha} and Hana {Druckmüllerová} and Miroslav {Jícha},
  title="Measurement of Fiber Deposition in a Human Lung Model by Phase Contrast Microscopy with Automated Image Analysis",
  year="2013",
  volume="20",
  number="3/4",
  month="December",
  pages="187--194",
  publisher="Association for Engineering Mechanics",
  address="Brno",
  issn="1802-1484"
}