Publication detail

Holographic confocal microscopy

CHMELÍK, R.

English title

Holographic confocal microscopy

Type

journal article - other

Language

en

Original abstract

Application of incoherent holography for achievement of the depth-discriminated microscopic imaging was proved. The image quality described by the coherent transfer function is comparable with that of a conventional confocal microscope; the imaging speed can be substantially higher. The observed plane is imaged as a whole, continuously in time, without any scanning system. Besides the amplitude, the phase of the confocal image is reconstructed, carrying information about phase shifts inside the specimen. The depth discrimination property can be enhanced beyond the capability of a conventional confocal microscope by the use of a broad-band illumination.

English abstract

Application of incoherent holography for achievement of the depth-discriminated microscopic imaging was proved. The image quality described by the coherent transfer function is comparable with that of a conventional confocal microscope; the imaging speed can be substantially higher. The observed plane is imaged as a whole, continuously in time, without any scanning system. Besides the amplitude, the phase of the confocal image is reconstructed, carrying information about phase shifts inside the specimen. The depth discrimination property can be enhanced beyond the capability of a conventional confocal microscope by the use of a broad-band illumination.

Keywords in English

confocal microscopy, incoherent holography, heterodyne microscopy, time gate

RIV year

2001

Released

01.01.2001

Publisher

SPIE - The International Society for Optical Engineering

Location

Washington

ISBN

0-8194-4047-7

ISSN

0277-786X

Book

Proceedings of SPIE

Volume

4356

Number

1

Edition number

1.

Pages count

6

BIBTEX


@article{BUT39897,
  author="Radim {Chmelík},
  title="Holographic confocal microscopy",
  booktitle="Proceedings of SPIE",
  year="2001",
  volume="4356",
  number="1",
  month="January",
  publisher="SPIE - The International Society for Optical Engineering",
  address="Washington",
  isbn="0-8194-4047-7",
  issn="0277-786X"
}