Publication detail
Steroid hormone-inducible biosensor based on EGFP-tagged and environmental application
Li, Zhonghe Gao, Xingai Li, Ming Yan, Qiuliang Zhang, Nan Yu, Boyang Zhang, Bimi Zhang, Shuying Helal, Mohamed H. Ali, Ola A. Abu Nassan, Mohamed A. Qyyum, Muhammad Abdul Asif, Saira Bokhari, Awais
English title
Steroid hormone-inducible biosensor based on EGFP-tagged and environmental application
Type
journal article in Web of Science
Language
en
Original abstract
Steroid hormones as a class of emerging organic pollutant and high concern, due to their potential risks for human and environmental. Accurate analytical methods of steroid hormones are necessary in quantifying and monitoring. Biosensor is a promising technique. In this study, though part of 3 alpha-HSD DNA to construct a regulatory plasmid and with the EGFP reporter gene to generate a reporter plasmid. Separately transformed into Escherichia coli strain BL21 and extracted the cell lysates as novel biosensor reagents. Analyzed the total amounts of steroid hormones in water, sediment, and soil samples using biosensor reagents, and compared these results with those obtained by HPLC. In summary, detection method using an EGFP reporter that can detect trace amounts of steroid hormones to reached fg/L. The optimal reaction time range and temperature were 30 min and 30 degrees C, respectively, while the most suitable organic solvent for the steroid hormone was 100% ethanol, up to 96well plate format. This method is very suitable for high-throughput detection of environmental steroid hormone pollutants.
English abstract
Steroid hormones as a class of emerging organic pollutant and high concern, due to their potential risks for human and environmental. Accurate analytical methods of steroid hormones are necessary in quantifying and monitoring. Biosensor is a promising technique. In this study, though part of 3 alpha-HSD DNA to construct a regulatory plasmid and with the EGFP reporter gene to generate a reporter plasmid. Separately transformed into Escherichia coli strain BL21 and extracted the cell lysates as novel biosensor reagents. Analyzed the total amounts of steroid hormones in water, sediment, and soil samples using biosensor reagents, and compared these results with those obtained by HPLC. In summary, detection method using an EGFP reporter that can detect trace amounts of steroid hormones to reached fg/L. The optimal reaction time range and temperature were 30 min and 30 degrees C, respectively, while the most suitable organic solvent for the steroid hormone was 100% ethanol, up to 96well plate format. This method is very suitable for high-throughput detection of environmental steroid hormone pollutants.
Keywords in English
Fluorescent biosensor; Steroid hormone; EGFP-Tagged; Sediment; Soil; Water; BACTERIAL BIOSENSORS; DRINKING-WATER; ESTROGENS; BIOAVAILABILITY; CONSTRUCTION; EXTRACTION; SENSORS; RIVER; ASSAY
Released
01.12.2022
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Location
San Diego
ISSN
0013-9351
Volume
2
Number
215
Pages count
7
BIBTEX
@article{BUT182642,
author="Saira {Asif} and Syed Awais Ali Shah {Bokhari},
title="Steroid hormone-inducible biosensor based on EGFP-tagged and environmental application",
year="2022",
volume="2",
number="215",
month="December",
publisher="ACADEMIC PRESS INC ELSEVIER SCIENCE",
address="San Diego",
issn="0013-9351"
}