Publication detail
Infrared Laser Desorption of Intact Nanoparticles for Digital Tissue Imaging
STIBOREK, M. JINDŘICHOVÁ, L. MELIORISOVÁ, S. BEDNAŘÍK, A. PRYSIAZHNYI, V. KROUPA, J. HOUŠKA, P. ADAMOVÁ, B. NAVRÁTILOVÁ, J. KANICKÝ, V. PREISLER, J.
English title
Infrared Laser Desorption of Intact Nanoparticles for Digital Tissue Imaging
Type
journal article in Web of Science
Language
en
Original abstract
We report a new technique for the digital mapping of biomarkers in tissues based on desorption and counting intact gold nanoparticle (Au NP) tags using infrared laser ablation single-particle inductively coupled plasma mass spectrometry (IR LA SP ICP MS). In contrast to conventional UV laser ablation, Au NPs are not disintegrated during the desorption process due to their low absorption at 2940 nm. A mass spectrometer detects up to 83% of Au NPs. The technique is demonstrated on mapping a proliferation marker, nuclear protein Ki-67, in three-dimensional (3D) aggregates of colorectal carcinoma cells, and the results are compared with confocal fluorescence microscopy and UV LA ICP MS. Precise counting of 20 nm Au NPs with a single-particle detection limit in each pixel by the new approach generates sharp distribution maps of a specific biomarker in the tissue. Advantageously, the desorption of Au NPs from regions outside the tissue is strongly suppressed. The developed methodology promises multiplex mapping of low-abundant biomarkers in numerous biological and medical applications using multielemental mass spectrometers.
English abstract
We report a new technique for the digital mapping of biomarkers in tissues based on desorption and counting intact gold nanoparticle (Au NP) tags using infrared laser ablation single-particle inductively coupled plasma mass spectrometry (IR LA SP ICP MS). In contrast to conventional UV laser ablation, Au NPs are not disintegrated during the desorption process due to their low absorption at 2940 nm. A mass spectrometer detects up to 83% of Au NPs. The technique is demonstrated on mapping a proliferation marker, nuclear protein Ki-67, in three-dimensional (3D) aggregates of colorectal carcinoma cells, and the results are compared with confocal fluorescence microscopy and UV LA ICP MS. Precise counting of 20 nm Au NPs with a single-particle detection limit in each pixel by the new approach generates sharp distribution maps of a specific biomarker in the tissue. Advantageously, the desorption of Au NPs from regions outside the tissue is strongly suppressed. The developed methodology promises multiplex mapping of low-abundant biomarkers in numerous biological and medical applications using multielemental mass spectrometers.
Keywords in English
Desorption; Fluorescence microscopy; Gold nanoparticles; Inductively coupled plasma mass spectrometry; Infrared lasers; Laser ablation; Mapping; Mass spectrometers; Medical applications; Tissue
Released
27.12.2022
Publisher
AMER CHEMICAL SOC
Location
WASHINGTON
ISSN
0003-2700
Volume
94
Number
51
Pages from–to
18114–18120
Pages count
7
BIBTEX
@article{BUT182555,
author="Marek {Stiborek} and Lenka {Jindřichová} and Stanislava {Meliorisová} and Antonín {Bednařík} and Vadym {Prysiazhnyi} and Jiří {Kroupa} and Pavel {Houška} and Barbora {Adamová} and Jarmila {Navrátilová} and Viktor {Kanický} and Jan {Preisler},
title="Infrared Laser Desorption of Intact Nanoparticles for Digital Tissue Imaging",
year="2022",
volume="94",
number="51",
month="December",
pages="18114--18120",
publisher="AMER CHEMICAL SOC",
address="WASHINGTON",
issn="0003-2700"
}