Publication detail

Analyses of viral genomes for G-quadruplex forming sequences reveal their correlation with the type of infection

BOHÁLOVÁ, N. CANTARA, A. BARTAS, M. KAURA, P. ŠŤASTNÝ, J. PEČINKA, P. FOJTA, M. MERGNY, J. BRÁZDA, V.

English title

Analyses of viral genomes for G-quadruplex forming sequences reveal their correlation with the type of infection

Type

journal article in Web of Science

Language

en

Original abstract

G-quadruplexes contribute to the regulation of key molecular processes. Their utilization for antiviral therapy is an emerging field of contemporary research. Here we present comprehensive analyses of the presence and localization of putative G-quadruplex forming sequences (PQS) in all viral genomes currently available in the NCBI database (including subviral agents). The G4Hunter algorithm was applied to a pool of 11,000 accessible viral genomes representing 350 Mbp in total. PQS frequencies differ across evolutionary groups of viruses, and are enriched in repeats, replication origins, 5′UTRs and 3′UTRs. Importantly, PQS presence and localization is connected to viral lifecycles and corresponds to the type of viral infection rather than to nucleic acid type; while viruses routinely causing persistent infections in Metazoa hosts are enriched for PQS, viruses causing acute infections are significantly depleted for PQS. The unique localization of PQS identifies the importance of G-quadruplex-based regulation of viral replication and life cycle, providing a tool for potential therapeutic targeting.

English abstract

G-quadruplexes contribute to the regulation of key molecular processes. Their utilization for antiviral therapy is an emerging field of contemporary research. Here we present comprehensive analyses of the presence and localization of putative G-quadruplex forming sequences (PQS) in all viral genomes currently available in the NCBI database (including subviral agents). The G4Hunter algorithm was applied to a pool of 11,000 accessible viral genomes representing 350 Mbp in total. PQS frequencies differ across evolutionary groups of viruses, and are enriched in repeats, replication origins, 5′UTRs and 3′UTRs. Importantly, PQS presence and localization is connected to viral lifecycles and corresponds to the type of viral infection rather than to nucleic acid type; while viruses routinely causing persistent infections in Metazoa hosts are enriched for PQS, viruses causing acute infections are significantly depleted for PQS. The unique localization of PQS identifies the importance of G-quadruplex-based regulation of viral replication and life cycle, providing a tool for potential therapeutic targeting.

Keywords in English

Acute infection; Bioinformatics; G-quadruplex; G4Hunter; Persistent infection; Viral genome

Released

01.07.2021

Publisher

Elsevier

ISSN

0300-9084

Volume

186

Number

1

Pages from–to

13–27

Pages count

15

BIBTEX


@article{BUT171738,
  author="Natália {Bohálová} and Alessio {Cantara} and Martin {Bartas} and Patrik {Kaura} and Jiří {Šťastný} and Petr {Pečinka} and Miroslav {Fojta} and Jean-Louis {Mergny} and Václav {Brázda},
  title="Analyses of viral genomes for G-quadruplex forming sequences reveal their correlation with the type of infection",
  year="2021",
  volume="186",
  number="1",
  month="July",
  pages="13--27",
  publisher="Elsevier",
  issn="0300-9084"
}