Publication detail
Influnce of Mutual Rotation of Polarizing Filters on Light Intensity Measured with Collagen Fibres
TURČANOVÁ, M. HRTOŇ, M. DVOŘÁK, P. BURŠA, J.
English title
Influnce of Mutual Rotation of Polarizing Filters on Light Intensity Measured with Collagen Fibres
Type
book chapter
Language
en
Original abstract
The most common technique for observation of collagen fibres and evaluation of their directionality is polarized light microscopy. In the microscope, there are two polarizing filters, polarizer and analyzer, that can rotate individually. The default position of the polarizer is perpendicular to the analyzer. When the light passes through a birefringent specimen, the plane of the polarized light is rotated. This leads to a 90◦ periodic change in light intensity passing through the sample and both filters. If the orientation of the polarizer differs from the perpendicular direction to the analyzer, the period of light intensity changes to 180◦; one of the original maxima increases and the other decreases. This change is very useful for the determination of directions of collagen fibres by an automatic algorithm.
English abstract
The most common technique for observation of collagen fibres and evaluation of their directionality is polarized light microscopy. In the microscope, there are two polarizing filters, polarizer and analyzer, that can rotate individually. The default position of the polarizer is perpendicular to the analyzer. When the light passes through a birefringent specimen, the plane of the polarized light is rotated. This leads to a 90◦ periodic change in light intensity passing through the sample and both filters. If the orientation of the polarizer differs from the perpendicular direction to the analyzer, the period of light intensity changes to 180◦; one of the original maxima increases and the other decreases. This change is very useful for the determination of directions of collagen fibres by an automatic algorithm.
Keywords in English
Polarized light microscopy; collagen fibers; birefrigence; light intensity
Released
28.09.2019
Publisher
Springer
Location
Porto, Portugalsko
ISBN
978-3-030-32040-9
Book
VipIMAGE 2019
Pages from–to
279–285
Pages count
7
BIBTEX
@inbook{BUT161919,
author="Michaela {Turčanová} and Martin {Hrtoň} and Petr {Dvořák} and Jiří {Burša},
title="Influnce of Mutual Rotation of Polarizing Filters on Light Intensity Measured with Collagen Fibres",
booktitle="VipIMAGE 2019
",
year="2019",
month="September",
pages="279--285",
publisher="Springer",
address="Porto, Portugalsko",
isbn="978-3-030-32040-9"
}