Publication detail

Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam

BEDNAŘÍK, A. KUBA, P. MOSKOVETS, E. TOMALOVÁ, I. KRÁSENSKÝ, P. HOUŠKA, P. PREISLER, J.

Czech title

Rychlá MALDI TOF spektrometrie se skenováním laserovým paprskem

English title

Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam

Type

journal article in Web of Science

Language

en

Original abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) imaging of surfaces and tissues is a rapidly evolving technique having great potential in the field of biosciences. In earlier times, acquisition of a single high-resolution MS image could take days. Despite the recent introduction of high-repetition rate lasers to increase sample throughput of axial TOF MS instruments, obtaining a high-resolution image still requires a few hours. This paper shows that a substantial increase in the throughput of the TOF MS-based tissue imaging can be achieved by incorporating a mirror providing high-speed precision scanning of the laser beam along the sample surface. Equipped with the scanning mirror, a laboratory-built axial MALDI TOF MS instrument utilizing a 4-kHz UV laser recorded a 100 x 100 pixel MS image in -11 min using 100 laser shots per pixel. This is almost an order of magnitude faster when compared to a modern commercial instrument equipped with 1-kHz laser.

Czech abstract

Skenování vzorků pomocí MALDI-TOF spektrometrie je rychle se vyvíjející technika, která má velký potenciál v oblasti biovědy. V dřívějších dobách trvalo kvalitní skenování velmi dlouho a to i přes použití nejnovějších laservých technologií. V tomto článku je představen koncept použití zrcátka na klonění laserového paprsku, což v důsledku vede k výraznému zrychlení celého procesu. Na prototypu se podařilo dosáhnout o řád nižších časů měření oproti komerčním výrobkům.

English abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) imaging of surfaces and tissues is a rapidly evolving technique having great potential in the field of biosciences. In earlier times, acquisition of a single high-resolution MS image could take days. Despite the recent introduction of high-repetition rate lasers to increase sample throughput of axial TOF MS instruments, obtaining a high-resolution image still requires a few hours. This paper shows that a substantial increase in the throughput of the TOF MS-based tissue imaging can be achieved by incorporating a mirror providing high-speed precision scanning of the laser beam along the sample surface. Equipped with the scanning mirror, a laboratory-built axial MALDI TOF MS instrument utilizing a 4-kHz UV laser recorded a 100 x 100 pixel MS image in -11 min using 100 laser shots per pixel. This is almost an order of magnitude faster when compared to a modern commercial instrument equipped with 1-kHz laser.

Keywords in English

mass spectrometry imaging; laser beam scanning; MALDI; TOF; high-throughput

RIV year

2014

Released

21.01.2014

Publisher

American Chemical Society

Location

Washington

ISSN

0003-2700

Volume

86

Number

2

Pages from–to

982–986

Pages count

5

BIBTEX


@article{BUT109667,
  author="Antonín {Bednařík} and Jan {Preisler} and Iva {Tomalová} and Eugene {MOSKOVETS} and Pavel {Krásenský} and Pavel {Houška} and Pavel {Kuba},
  title="Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam",
  year="2014",
  volume="86",
  number="2",
  month="January",
  pages="982--986",
  publisher="American Chemical Society",
  address="Washington",
  issn="0003-2700"
}