Publication detail
Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam
BEDNAŘÍK, A. KUBA, P. MOSKOVETS, E. TOMALOVÁ, I. KRÁSENSKÝ, P. HOUŠKA, P. PREISLER, J.
Czech title
Rychlá MALDI TOF spektrometrie se skenováním laserovým paprskem
English title
Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam
Type
journal article in Web of Science
Language
en
Original abstract
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) imaging of surfaces and tissues is a rapidly evolving technique having great potential in the field of biosciences. In earlier times, acquisition of a single high-resolution MS image could take days. Despite the recent introduction of high-repetition rate lasers to increase sample throughput of axial TOF MS instruments, obtaining a high-resolution image still requires a few hours. This paper shows that a substantial increase in the throughput of the TOF MS-based tissue imaging can be achieved by incorporating a mirror providing high-speed precision scanning of the laser beam along the sample surface. Equipped with the scanning mirror, a laboratory-built axial MALDI TOF MS instrument utilizing a 4-kHz UV laser recorded a 100 x 100 pixel MS image in -11 min using 100 laser shots per pixel. This is almost an order of magnitude faster when compared to a modern commercial instrument equipped with 1-kHz laser.
Czech abstract
Skenování vzorků pomocí MALDI-TOF spektrometrie je rychle se vyvíjející technika, která má velký potenciál v oblasti biovědy. V dřívějších dobách trvalo kvalitní skenování velmi dlouho a to i přes použití nejnovějších laservých technologií. V tomto článku je představen koncept použití zrcátka na klonění laserového paprsku, což v důsledku vede k výraznému zrychlení celého procesu. Na prototypu se podařilo dosáhnout o řád nižších časů měření oproti komerčním výrobkům.
English abstract
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) imaging of surfaces and tissues is a rapidly evolving technique having great potential in the field of biosciences. In earlier times, acquisition of a single high-resolution MS image could take days. Despite the recent introduction of high-repetition rate lasers to increase sample throughput of axial TOF MS instruments, obtaining a high-resolution image still requires a few hours. This paper shows that a substantial increase in the throughput of the TOF MS-based tissue imaging can be achieved by incorporating a mirror providing high-speed precision scanning of the laser beam along the sample surface. Equipped with the scanning mirror, a laboratory-built axial MALDI TOF MS instrument utilizing a 4-kHz UV laser recorded a 100 x 100 pixel MS image in -11 min using 100 laser shots per pixel. This is almost an order of magnitude faster when compared to a modern commercial instrument equipped with 1-kHz laser.
Keywords in English
mass spectrometry imaging; laser beam scanning; MALDI; TOF; high-throughput
RIV year
2014
Released
21.01.2014
Publisher
American Chemical Society
Location
Washington
ISSN
0003-2700
Volume
86
Number
2
Pages from–to
982–986
Pages count
5
BIBTEX
@article{BUT109667,
author="Antonín {Bednařík} and Jan {Preisler} and Iva {Tomalová} and Eugene {MOSKOVETS} and Pavel {Krásenský} and Pavel {Houška} and Pavel {Kuba},
title="Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam",
year="2014",
volume="86",
number="2",
month="January",
pages="982--986",
publisher="American Chemical Society",
address="Washington",
issn="0003-2700"
}