Publication detail

Suitable Technological Conditions for Enzymatic Hydrolysis of Waste Paper by Novozymes Enzymes NS50013 and NS50010

BRUMMER, V. SKRYJA, P. JUŘENA, T. HLAVÁČEK, V. STEHLÍK, P.

Czech title

Vhodné technologické podmínky pro enzymatickou hydrolýzu odpadního papíru enzymy Novozymes NS50013 a NS50010

English title

Suitable Technological Conditions for Enzymatic Hydrolysis of Waste Paper by Novozymes Enzymes NS50013 and NS50010

Type

journal article in Web of Science

Language

en

Original abstract

Waste paper belongs to a group of quantitatively the most produced waste types. Enzymatic hydrolysis is becoming a suitable way to treat this type of waste and at the same time, to produce a valuable liquid biofuel, because reducing sugars solutions that are formed during the process of saccharification can be a precursor for following or simultaneous fermentation. If it will be possible to make the enzymatic hydrolysis of the waste paper economically viable, it could serve as one of the new ways to lower the dependence of the transport sector on oil in the future. Only several studies comparing the enzymatic hydrolysis of different waste papers were performed in the past; they are summarized in this manuscript. In our experimental trials, suitable technological conditions for waste paper enzymatic hydrolysis using enzymes from Novozymes biomass kit: enzymes NS50013 and NS50010 were investigated. The following enzymatic hydrolysis parameters in laboratory scale trials were verified on high cellulose content substrates–filter paper and cellulose pulp: type of buffer, pH, temperature, concentration of the substrate, loading of the enzyme and rate of stirring.

Czech abstract

Odpadní papír patří do skupiny kvantitativně nejvíce produkovaných typů odpadů. Enzymatická hydrolýza se stává vhodným způsobem, jak zacházet s tímto druhem odpadu a současně produkovat cenné kapalné biopalivo, protože roztoky redukujících cukrů, které jsou vytvořeny během procesu sacharifikace mohou být prekurzorem pro následující nebo simultánní fermentaci. V případě že bude možné učinit enzymatickou hydrolýzu papírového odpadu ekonomicky životaschopnou, mohla by sloužit jako jeden z nových způsobů, jak snížit závislost transportního sektoru na ropě v budoucnu. Pouze několik studií srovnávajících enzymatickou hydrolýzu různých odpadních papírů byly provedeny v minulosti; tyto jsou shrnuty v tomto článku. V našich experimentálních zkouškách, byly hledány vhodné technologické podmínky pro enzymatickou hydrolýzu odpadního papíru pomocí enzymů ze sady Novozymes biomass kit: enzymy NS50013 a NS50010. Následující enzymatické parametry hydrolýzy byly ověřovány v laboratorním měřítku na substrátech s vysokým obsahem celulózy, filtrační papír a celulózové buničiny: typ pufru, pH, teplota, koncentrace substrátu, koncentrace enzymu a rychlost míchání.

English abstract

Waste paper belongs to a group of quantitatively the most produced waste types. Enzymatic hydrolysis is becoming a suitable way to treat this type of waste and at the same time, to produce a valuable liquid biofuel, because reducing sugars solutions that are formed during the process of saccharification can be a precursor for following or simultaneous fermentation. If it will be possible to make the enzymatic hydrolysis of the waste paper economically viable, it could serve as one of the new ways to lower the dependence of the transport sector on oil in the future. Only several studies comparing the enzymatic hydrolysis of different waste papers were performed in the past; they are summarized in this manuscript. In our experimental trials, suitable technological conditions for waste paper enzymatic hydrolysis using enzymes from Novozymes biomass kit: enzymes NS50013 and NS50010 were investigated. The following enzymatic hydrolysis parameters in laboratory scale trials were verified on high cellulose content substrates–filter paper and cellulose pulp: type of buffer, pH, temperature, concentration of the substrate, loading of the enzyme and rate of stirring.

Keywords in Czech

Enzymatická hydrolýza, bioetanol, odpadní papír, Novozymes, technologické podmínky, enzymy, celuláza, celulóza, NS50013 , NS50010

Keywords in English

Enzymatic hydrolysis , Bioethanol , Waste paper , Novozymes , Technological conditions , Enzymes , Cellulase , Cellulose , NS50013 , NS50010

RIV year

2014

Released

08.08.2014

Publisher

Springer Science + Business Media

Location

New York

ISSN

0273-2289

Volume

174

Number

4

Pages from–to

1299–1308

Pages count

10

BIBTEX


@article{BUT109378,
  author="Vladimír {Brummer} and Pavel {Skryja} and Tomáš {Juřena} and Viliam {Hlaváček} and Petr {Stehlík},
  title="Suitable Technological Conditions for Enzymatic Hydrolysis of Waste Paper by Novozymes Enzymes NS50013 and NS50010",
  year="2014",
  volume="174",
  number="4",
  month="August",
  pages="1299--1308",
  publisher="Springer Science + Business Media",
  address="New York",
  issn="0273-2289"
}