Mapování distribuce vybraných prvků v rostlinách pomocí LIBS

Mapping of elemental distribution in plant samples by Laser-Induced Breakdown Spectroscopy (LIBS)

abstrakt

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Laser Induced Breakdown Spectroscopy (LIBS) is a powerful tool for scanning an elemental spatial distribution within different analytes. Higher sensitivity of this method can be reached in double-pulse configuration (DP LIBS), in which the microplasma is re-heated with another laser pulse. One of the possible applications of LIBS can be the mapping of plant compartments, where the elemental distribution within different tissues and organs could be observed. Moreover, recently the monitoring of heavy metal pollution, together with environmentally friendly cleaning of polluted sites also receives an increased interest. For polluted soils different remediation possibilities exist. One of them is the phytoremediation, a process which uses plants to accumulate heavy metals from soil. Mapping of these metals in plant organs and tissues is important both, for protecting the human health and for controlling and improving the phytoremediation processes. Here we report on analysis of tree compartments (conifer-needles, twigs, leaves) grown at remediation area of former copper mine. Line scans and 2D maps of copper and calcium distribution were obtained by LIBS analysis. For comparative laboratory experiments spruce (Picea abies) samples were chosen. Cross sections throughout annual terminal twigs cultivated in CuCl2 solution of different concentrations were prepared and analyzed by DP LIBS. Raster scanning with 150 um spatial resolution was set and 2D maps from data were created. Some twig parts originated in the vicinity of the implementation of cross sections were mineralized and subsequently the Cu and Ca content by inductively coupled plasma mass spectrometry (ICP-MS) were analyzed. The results give quantitative information about overall concentration of elements in places where the LIBS measurements were realized. According to the determined contents the LIBS spectra (and the maps created from these spectra) were normalized.

Spektrometrie laserem indukovaného mikroplazmatu je analytická metoda která může být modifikována k prvkové detekci pevných roztoků. V práci je popsána aplikace LIBS na prvkové mapování, tj. na studium rozložení vybraných prvků v různých částech rostlin.

Laser Induced Breakdown Spectroscopy (LIBS) is a powerful tool for scanning an elemental spatial distribution within different analytes. Higher sensitivity of this method can be reached in double-pulse configuration (DP LIBS), in which the microplasma is re-heated with another laser pulse. One of the possible applications of LIBS can be the mapping of plant compartments, where the elemental distribution within different tissues and organs could be observed. Moreover, recently the monitoring of heavy metal pollution, together with environmentally friendly cleaning of polluted sites also receives an increased interest. For polluted soils different remediation possibilities exist. One of them is the phytoremediation, a process which uses plants to accumulate heavy metals from soil. Mapping of these metals in plant organs and tissues is important both, for protecting the human health and for controlling and improving the phytoremediation processes. Here we report on analysis of tree compartments (conifer-needles, twigs, leaves) grown at remediation area of former copper mine. Line scans and 2D maps of copper and calcium distribution were obtained by LIBS analysis. For comparative laboratory experiments spruce (Picea abies) samples were chosen. Cross sections throughout annual terminal twigs cultivated in CuCl2 solution of different concentrations were prepared and analyzed by DP LIBS. Raster scanning with 150 um spatial resolution was set and 2D maps from data were created. Some twig parts originated in the vicinity of the implementation of cross sections were mineralized and subsequently the Cu and Ca content by inductively coupled plasma mass spectrometry (ICP-MS) were analyzed. The results give quantitative information about overall concentration of elements in places where the LIBS measurements were realized. According to the determined contents the LIBS spectra (and the maps created from these spectra) were normalized.

LIBS, ns, plant compartments, mapping

02.10.2011

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