The 2-D and 3-D processing of images provided by conventional microscopes

článek v časopise - ostatní, Jost

en

The paper presents an interesting processing method of microscopic images. High-pass type filters are generally used for image focusing. They enhance the high spatial frequencies. They are, however, efficient only in cases, when the picture is unsharp because of the low contrast on high frequencies (for example in a TV picture). These filters, are not appropriate if the unsharpness has been caused by other factors. In this case, it is not possible to construct a 3-D model of the observed object. We can obtain better results and a 3-D model by applying the following theory. As part of this paper, an original program based on this theory is described.

The paper presents an interesting processing method of microscopic images. High-pass type filters are generally used for image focusing. They enhance the high spatial frequencies. They are, however, efficient only in cases, when the picture is unsharp because of the low contrast on high frequencies (for example in a TV picture). These filters, are not appropriate if the unsharpness has been caused by other factors. In this case, it is not possible to construct a 3-D model of the observed object. We can obtain better results and a 3-D model by applying the following theory. As part of this paper, an original program based on this theory is described.

graphic plane, physical pixel, multi-focal image, Euclidean trace of point, focusing criterion

2003

01.01.2002

SCANNING

2002

6/2002

13