Detail publikace
MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second
BEDNAŘÍK, A. MACHÁLKOVÁ, M. MOSKOVETS, E. COUFALÍKOVÁ, K. KRÁSENSKÝ, P. HOUŠKA, P. KROUPA, J. NAVRÁTILOVÁ, J. ŠMARDA, J. PREISLER, J.
Anglický název
MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
en
Originální abstrakt
The practicality of matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) applied to molecular imaging of biological tissues is limited by the analysis speed. Typically, a relatively low speed of stop-and-go micromotion of XY stages is considered as a factor substantially reducing the rate with which fresh sample material can be supplied to the laser spot. The sample scan rate in our laboratory-built high-throughput imaging TOF mass spectrometer was significantly improved through the use of a galvanometer-based optical scanner performing fast laser spot repositioning on a target plate. The optical system incorporated into the ion source of our MALDI TOF mass spectrometer allowed focusing the laser beam via a modified grid into a 10-m round spot. This permitted the acquisition of high-resolution MS images with a well-defined pixel size at acquisition rates exceeding 100pixel/s. The influence of selected parameters on the total MS imaging time is discussed. The new scanning technique was employed to display the distribution of an antitumor agent in 3D colorectal adenocarcinoma cell aggregates; a single MS image comprising 100x100pixels with 10-m lateral resolution was recorded in approximately 70s. [GRAPHICS] .
Anglický abstrakt
The practicality of matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) applied to molecular imaging of biological tissues is limited by the analysis speed. Typically, a relatively low speed of stop-and-go micromotion of XY stages is considered as a factor substantially reducing the rate with which fresh sample material can be supplied to the laser spot. The sample scan rate in our laboratory-built high-throughput imaging TOF mass spectrometer was significantly improved through the use of a galvanometer-based optical scanner performing fast laser spot repositioning on a target plate. The optical system incorporated into the ion source of our MALDI TOF mass spectrometer allowed focusing the laser beam via a modified grid into a 10-m round spot. This permitted the acquisition of high-resolution MS images with a well-defined pixel size at acquisition rates exceeding 100pixel/s. The influence of selected parameters on the total MS imaging time is discussed. The new scanning technique was employed to display the distribution of an antitumor agent in 3D colorectal adenocarcinoma cell aggregates; a single MS image comprising 100x100pixels with 10-m lateral resolution was recorded in approximately 70s. [GRAPHICS] .
Klíčová slova anglicky
Mass spectrometry imaging; Laser beam scanning; MALDI; TOF; MSI; High throughput; Grid ion source; 3D cell aggregates; Spheroids; Colorectal adenocarcinoma
Vydáno
15.02.2019
Nakladatel
SPRINGER
Místo
NEW YORK
ISSN
1044-0305
Ročník
30
Číslo
2
Strany od–do
289–298
Počet stran
10
BIBTEX
@article{BUT162696,
author="Antonín {Bednařík} and Markéta {Machálková} and Eugene {MOSKOVETS} and Kateřina {Coufalíková} and Pavel {Krásenský} and Pavel {Houška} and Jiří {Kroupa} and Jarmila {Navrátilová} and Jan {Šmarda} and Jan {Preisler},
title="MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second",
year="2019",
volume="30",
number="2",
month="February",
pages="289--298",
publisher="SPRINGER",
address="NEW YORK",
issn="1044-0305"
}