ELECTROMAGNET FOR CAPTURE OF MAGNETIC BEADS FROM LARGE VOLUME INTO MICROFLUIDIC CHAMBRE CHIP

článek ve sborníku ve WoS nebo Scopus

en

Immunomagnetic separation is method of choice besides filtration or centrifugation when a bioanalyte is to be specifically isolated form a larger volume. Here, home-made electromagnet with special, optimized construction, to fit to the microfluidic rhombic chip is presented. The experiments show how to capture 1 mg of superparamagnetic beads (SMB) from 20 mL syringe into 120 µL chamber in microfluidic chip. The captured beads can be easily transported to another microfluidic device for subsequent analysis. Various flow rates were tested and SMB were collected firstly from a phosphate buffer and subsequently from a cow's milk sample.

Immunomagnetic separation is method of choice besides filtration or centrifugation when a bioanalyte is to be specifically isolated form a larger volume. Here, home-made electromagnet with special, optimized construction, to fit to the microfluidic rhombic chip is presented. The experiments show how to capture 1 mg of superparamagnetic beads (SMB) from 20 mL syringe into 120 µL chamber in microfluidic chip. The captured beads can be easily transported to another microfluidic device for subsequent analysis. Various flow rates were tested and SMB were collected firstly from a phosphate buffer and subsequently from a cow's milk sample.

electromagnet, magnetic beads, microfluidic, chip

16.10.2018

Institute of Analytical Chemistry of the CAS

Brno

978-80-904959-5-1

CECE 2018

286–289

4