Detail publikace
Konečných prvků simulace mechanických zkoušek s modely bendo tensegrity hladké svalové buňky.
BANSOD, Y. BURŠA, J.
Český název
Konečných prvků simulace mechanických zkoušek s modely bendo tensegrity hladké svalové buňky.
Anglický název
Finite element simulation of mechanical tests with bendo-tensegrity models of smooth muscle cell.
Typ
abstrakt
Jazyk
en
Originální abstrakt
It is now evident that microtubules do not have compression-only behavior but appear highly curved in living cells under no external load. This indicates that the compressive forces in them are accompanied by significant bending even if loaded solely by the prestrained actin bundles; this is referred to as the “bendo-tensegrity” concept. Implementing this concept, finite element models of smooth muscle cell (SMC) are proposed considering the dominating role of flexion in behavior of microtubules. A Finite element simulation of atomic force microscopy (AFM) experiment with adherent cell model and tensile test with suspended cell model is performed. The numerically predicted force-displacement curves of both simulations are analogous to the non-linear experimental responses obtained with AFM and tensile test for SMC; this validates the proposed bendo-tensegrity cell models. Results show that the actin cortex plays a vital role in maintaining the cell rigidity under local deformation, whereas the microfilaments, microtubules and intermediate filaments contribute significantly to the cell response under global deformation.
Český abstrakt
Nově vytvořený model je charakteristický zakřiveným tvarem mikrotubulů, které tak jsou namáhány ohybem namísto tahu-tlaku a tudíž jsou mnohem poddajnější. Tím je dosaženo jejich mnohem realističtějšího příspěvku k výsledné tuhosti buňky při různých způsobech mechanického namáhání.
Anglický abstrakt
It is now evident that microtubules do not have compression-only behavior but appear highly curved in living cells under no external load. This indicates that the compressive forces in them are accompanied by significant bending even if loaded solely by the prestrained actin bundles; this is referred to as the “bendo-tensegrity” concept. Implementing this concept, finite element models of smooth muscle cell (SMC) are proposed considering the dominating role of flexion in behavior of microtubules. A Finite element simulation of atomic force microscopy (AFM) experiment with adherent cell model and tensile test with suspended cell model is performed. The numerically predicted force-displacement curves of both simulations are analogous to the non-linear experimental responses obtained with AFM and tensile test for SMC; this validates the proposed bendo-tensegrity cell models. Results show that the actin cortex plays a vital role in maintaining the cell rigidity under local deformation, whereas the microfilaments, microtubules and intermediate filaments contribute significantly to the cell response under global deformation.
Klíčová slova česky
Cytoskelet, Bendo-tensegrity, metodou konečných prvků, modelování, buňka mechaniky
Klíčová slova anglicky
Cytoskeleton, Bendo-tensegrity, FInite element modelling, Cell mechanics
Vydáno
18.05.2016
Nakladatel
XXIV Cytoskeletal Club, Veterinary Research Institute, Masaryk University.
Místo
Vranovska Ves, Czech Republic.