Detail publikace
Porovnání metod pro vyhodnocení depozice částic v modelu lidských plic
BĚLKA, M. LIPPAY, J. LÍZAL, F. JEDELSKÝ, J. JÍCHA, M.
Český název
Porovnání metod pro vyhodnocení depozice částic v modelu lidských plic
Anglický název
Comparison of methods for evaluation of aerosol deposition in the model of human lungs
Typ
článek ve sborníku ve WoS nebo Scopus
Jazyk
en
Originální abstrakt
It seems to be very convenient to receive a medicine by inhalation instead of injection. Unfortunately transport of particles and targeted delivery of a drug in human respiratory airways is very complicated task. Therefore we carried out experiments and tested different methods for evaluation of particle deposition in a model of human lungs. The model included respiratory airways from oral cavity to 7th generation of branching. Particles were dispersed by TSI Small-scale Powder Disperser 3433 and delivered to the model. The model was disassembled into segments after the deposition of the particles and local deposition was measured. Two methods were used to analyse the samples, fluorescence spectroscopy and optical microscopy. The first method was based on measuring the intensity of luminescence, which represented the particle deposition. The second method used the optical microscope with phase-contrast objective. A dispersion of isopropanol and particles was filtrated using a vacuum filtration unit, a filter was placed on glass slide and made transparent. The particles on the filter were counted manually and the deposition was calculated afterwards. The results of the methods were compared and both methods proved to be useful.
Český abstrakt
Podávání léků inhalací místo injekční cestou je velice pohodlné. Avšak transport aerosolů a jeho cílená dodávka v lidských dýchacích cestách je velice komplikovaný problém. Proto bylo provedeno několik depozičních experimentů a odzkoušeno několik metod pro vyhodnocení depozice v modelu lidských plic. Model obsahoval dýchací cesty od ústní dutiny až po 7. generaci větvení. Částice byly dispergovány pomocí TSI SSPD 3433 a aplikovány do modelu. Po ukončení depozice byl model rozebrán a depozice v jednotlivých částech byla analyzována. Pro vyhodnocení byly použity 2 metody, fluorescenční spektroskopie a optická mikroskopie. První metoda využívá měření intenzity fluorescence částic, jejíž velikost určuje míru depozice. Druhá metoda využívá optické mikroskopie ve spojení s fázovým kontrastem. Výsledky obou metod byly porovnány a metody byly vyhodnoceny jako použitelné.
Anglický abstrakt
It seems to be very convenient to receive a medicine by inhalation instead of injection. Unfortunately transport of particles and targeted delivery of a drug in human respiratory airways is very complicated task. Therefore we carried out experiments and tested different methods for evaluation of particle deposition in a model of human lungs. The model included respiratory airways from oral cavity to 7th generation of branching. Particles were dispersed by TSI Small-scale Powder Disperser 3433 and delivered to the model. The model was disassembled into segments after the deposition of the particles and local deposition was measured. Two methods were used to analyse the samples, fluorescence spectroscopy and optical microscopy. The first method was based on measuring the intensity of luminescence, which represented the particle deposition. The second method used the optical microscope with phase-contrast objective. A dispersion of isopropanol and particles was filtrated using a vacuum filtration unit, a filter was placed on glass slide and made transparent. The particles on the filter were counted manually and the deposition was calculated afterwards. The results of the methods were compared and both methods proved to be useful.
Klíčová slova česky
Depozice aerosolu, optická mikroskopie, fluorescenční spektroskopie, dýchací cesty
Klíčová slova anglicky
Aerosol deposition, optical microscopy, fluorescent spectroscopy, human lungs
Rok RIV
2013
Vydáno
25.03.2014
Nakladatel
EDP Sciences
Místo
FRANCE
ISBN
978-80-260-5375-0
ISSN
2100-014X
Kniha
EPJ Web of Conferences
Ročník
67
Číslo
1
Strany od–do
82–85
Počet stran
4
BIBTEX
@inproceedings{BUT104093,
author="Miloslav {Bělka} and Josef {Lippay} and František {Lízal} and Jan {Jedelský} and Miroslav {Jícha},
title="Comparison of methods for evaluation of aerosol deposition in the model of human lungs",
booktitle="EPJ Web of Conferences",
year="2014",
volume="67",
number="1",
month="March",
pages="82--85",
publisher="EDP Sciences",
address="FRANCE",
isbn="978-80-260-5375-0",
issn="2100-014X"
}